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diff --git a/biblio/8534373.mdwn b/biblio/8534373.mdwn
new file mode 100644 (file)
index 0000000..dcc8c29
--- /dev/null
+++ b/biblio/8534373.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Transcription of the 5'-terminal cap nucleotide by RNA-dependent DNA polymerase: possible involvement in retroviral reverse transcription."]]
+[[!tag reverse_transcriptase cap]]
+
+Volloch VZ, Schweitzer B, Rits S.
+
+DNA Cell Biol. 1995 Dec;14(12):991-6 doi:10.1089/dna.1995.14.991
+
+Transcription of the 5'-terminal cap nucleotide by RNA-dependent DNA polymerase: possible involvement in retroviral reverse transcription.
+
+[[!pmid 8534373 desc="Utilises a 5′ hairpin in the beta-globin mRNA to design a cDNA extension assay where the reverse-transcribed cap creates a terminal mismatch that inhibits extension.  De-capping the RNA removes the inhibition.  40 mM Tris 8.3, 40 mM KCl, 5 mM MgCl2, 2 mM DTT.  AMV, 45°C"]]

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 51efdcd8f5acc760d001613deef9e7ebab897fb8..3e3d5b97995b0b63039570527720634d9926aba3 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -79,8 +79,9 @@ nucleotide as a template.
    at the 5′ end of cDNA clones, and concluded that the cap can be
    reverse-transcribed.  They supported their conclusion with molecular modelling.
 
- - [[Volloch et al, 1995|biblio/8534373]] studied cap transcription (but I could
-   not access the article).
+ - [[Volloch, Schweitzer and Rits, 1995|biblio/8534373]] showed that the AMV
+   reverse-transcribes the cap, using an assay where the extension of a natural
+   hairpin in the beta-globin cDNA can be enabled by decapping.
 
  - [[Kulpa, Topping and Telesnitsky, 1997|biblio/9049314]] noted the addition
    of one G at the position where the 5′ end of the MMLV RNA was