RT specificity

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+[[!meta title="Detection of splice isoforms and rare intermediates using multiplexed primer extension sequencing."]]
+[[!tag reverse_transcription method]]
+
+Nat Methods. 2019 Jan;16(1):55-58. doi:10.1038/s41592-018-0258-x
+
+Xu H, Fair BJ, Dwyer ZW, Gildea M, Pleiss JA.
+
+Detection of splice isoforms and rare intermediates using multiplexed primer extension sequencing.
+
+[[!pmid 30573814 desc="Specificity of RT priming can be increased by increasing temperature from 47 °C to 55 °C."]]