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"Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2020-06-15 07:45+0000\n"
+"POT-Creation-Date: 2020-06-15 07:48+0000\n"
"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
"Language-Team: LANGUAGE <LL@li.org>\n"
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-"My training as a researcher started with developmental genetics in "
-"drosophila and zebrafish, where I studied the activity of transcription "
+"My training as a researcher started with **developmental genetics in "
+"drosophila and zebrafish**, where I studied the activity of transcription "
"enhancers ([Blader and coll., 2003](https://pubmed.gov/12559493)) and their "
"evolutionary conservation (Plessy et al., 2005). This gave me a strong "
"interest for whole-transcriptome analysis and technology. For that purpose, "
"I have joined RIKEN in 2004, where have worked on high-throughput methods "
-"for profiling promoters and inferring gene networks, and in particular on "
-"CAGE (Cap Analysis Gene Expression)."
+"for **profiling promoters and inferring gene networks**, and in particular "
+"on CAGE (Cap Analysis Gene Expression)."
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-"I have developed a miniaturized version of CAGE, termed nanoCAGE, to analyse "
-"small samples yielding only nanograms of RNA (Plessy et al., 2010). In the "
-"same manuscript, we also introduced its paired-end variant, CAGEscan, which "
-"we use to associate novel promoters with annotations. Since then, we have "
-"kept improving or expanding these techniques, by updating the protocol "
-"(Salimullah et al., 2011), reducing the sequence bias introduced by the "
-"molecular barcodes (Tang et al., 2013), combining multiple cap-enrichment "
-"steps (Batut et al., 2013), benchmarking the use of locked nucleic acids for "
-"template switching (Harbers et al., 2013), and reducing the number of primer "
-"artefacts and unwanted sequences generated by ribosomal RNAs using low-"
-"complexity “pseudo-random” reverse-transcription primers (Arnaud et al., "
-"2016)."
+"I have developed a miniaturized version of CAGE, termed **nanoCAGE**, to "
+"analyse small samples yielding only nanograms of RNA (Plessy et al., 2010). "
+"In the same manuscript, we also introduced its paired-end variant, "
+"**CAGEscan**, which we use to **associate novel promoters with "
+"annotations**. Since then, we have kept improving or expanding these "
+"techniques, by updating the protocol (Salimullah et al., 2011), reducing the "
+"sequence bias introduced by the molecular barcodes (Tang et al., 2013), "
+"combining multiple cap-enrichment steps (Batut et al., 2013), benchmarking "
+"the use of locked nucleic acids for template switching (Harbers et al., "
+"2013), and reducing the number of primer artefacts and unwanted sequences "
+"generated by ribosomal RNAs using low-complexity “pseudo-random” reverse-"
+"transcription primers (Arnaud et al., 2016)."
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"On April 2013, I started a new development cycle as the leader of the "
"Genomics Miniaturization Technology Unit at RIKEN Center for Life Sciences, "
"Division of Genomics Technology, to expand this work on single cells "
-"following a population transcriptomics approach (Plessy et al., 2013) "
+"following a **population transcriptomics** approach (Plessy et al., 2013) "
"focused on sampling the largest possible number of cells. In our ongoing "
-"developments, we have reached single-cell and single molecule resolution "
+"developments, we have reached **single-cell and single molecule resolution** "
"through the introduction of transposase fragmentation and unique molecular "
"identifiers (Poulain et al., 2017). The protocol exists in two versions, one "
"for FACS-isolated cells, and one for the Fluidigm C1 platform (Kouno et al., "
"endogenous promoter, I combined Deep-RACE, CAGE and paired-end sequencing in "
"a technology that we called “Single-Locus CAGE” (Haberle et al., 2014). With "
"my contributions related to CAGE development and analysis, I have been a "
-"member of the FANTOM consortium since FANTOM3."
+"**member of the FANTOM consortium** since FANTOM3."
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"Together with my colleagues at RIKEN and collaborators in the field of "
"neuroscience, I have applied nanoCAGE to the study of single neuron cell "
-"types, for instance the olfactory neurons (Plessy et al., 2012), or in "
+"types, for instance the **olfactory neurons** (Plessy et al., 2012), or in "
"dopaminergic cells, where we could demonstrate the expression of haemoglobin "
"in the midbrain (Biagioli et al., 2009). We are also exploring the sub-"
-"cellular localisation of RNA in Purkinje neurons (Kratz et al., 2014), and "
-"neurogenesis in the mouse olfactory epithelium using single-cell CAGE and "
-"ATAC-seq techniques. In parallel with this promoter-centric work, I have "
-"also explored the huge repertoire of the T cell antigen receptors."
+"cellular localisation of RNA in **Purkinje neurons** (Kratz et al., 2014), "
+"and neurogenesis in the mouse olfactory epithelium using single-cell CAGE "
+"and ATAC-seq techniques. In parallel with this promoter-centric work, I have "
+"also explored the huge repertoire of the **T cell antigen receptors**."
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-"I joined OIST in 2018, to study the genetic structure and population "
-"variations of an animal plankton, Oikopleura dioica, that has a genome 50 "
+"I joined OIST in 2018, to study **the genetic structure and population "
+"variations** of an animal plankton, Oikopleura dioica, that has a genome 50 "
"time more compact than the human one, which empowers us to sequence at "
"chromosomal resolution many individual sampled from all over the World."
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-"I am also a Free Software enthusiast, and contribute to the Debian Med "
-"project, by packaging bioinformatics tools, which are redistributed in "
+"I am also a **Free Software** enthusiast, and contribute to the Debian Med "
+"project, by **packaging bioinformatics tools**, which are redistributed in "
"Debian (Möller et al., 2010) and its derivatives such as Ubuntu and "
"(cloud)Bio-Linux. For digital signature of my contributions and other "
"activities as a RIKEN researcher, I use the GPG key number B3443334. My "
projects / source.git / commitdiff