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+[[!meta title="Concerted cutting by Spo11 illuminates meiotic DNA break mechanics."]]
+[[!tag meiosis repair]]
+
+Johnson D, Crawford M, Cooper T, Claeys Bouuaert C, Keeney S, Llorente B, Garcia V, Neale MJ.
+
+Concerted cutting by Spo11 illuminates meiotic DNA break mechanics.
+
+Nature. 2021 Jun;594(7864):572-576. doi:10.1038/s41586-021-03389-3
+
+[[!pmid 34108687 desc="Pairs of double strand breaks created by Spo11 causes loss of short oligonucleotides, creating a cap repaired by homologous recombination."]]
+
+“High-resolution analysis of deproteinized Spo11-oligos showed [a ladder of] periodicity of around 10 nt [that] ranges in length from around 33 nt to more than 100 nt”  “The ladder also arose in rad50S-mutant and Mre11-nuclease-defective strains of S. cerevisiae, which, like the sae2∆ mutant, cannot remove Spo11 from DSB ends. Notably, the ladder depended on the catalytic activity of Spo11”  “we refer to this ladder as hyperlocalized ‘Spo11 double cuts’ (Spo11-DCs).”
+
+“because Spo11-DCs of less than 30 nt in length are not detected on gels and are depleted from filtered Spo11-oligo libraries, we propose that adjacent Spo11 complexes that are capable of making double cuts must, in vivo, interact with DNA from the same direction, thereby generating a ladder of Spo11-DCs with periodicity dictated by the helical pitch of DNA (around 10.5 bp).”
+
+“the global frequency of Spo11-DCs was disproportionately associated with regions of stronger Spo11 activity”
+
+“Deep sequencing of meiotic progeny identifies recombination scars that are consistent with repair initiated from gaps generated by adjacent Spo11 DSBs.”
+
+“We envision a mechanism in which multiple Spo11 proteins assemble with other pro-DSB factors to create a platform that enables concerted Spo11-DSB formation”

diff --git a/tags/meiosis.mdwn b/tags/meiosis.mdwn
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 [[!meta title="pages tagged meiosis"]]
 
-[[!inline pages="tagged(meiosis)" actions="no" archive="yes"
-feedshow=10]]
+_in progress_
+
+ - Spo11 cleaves both DNA strands.  Double cleavage creates gaps repaired by
+   homologous recombination ([[Johnson and coll., 2021|biblio/34108687]]).
+
+[[!inline pages="tagged(meiosis)" limit=0]]