Nouveaux articles et consolidations sur cellules isolées.

diff --git a/biblio/12202777.mdwn b/biblio/12202777.mdwn
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+[[!meta title="Improved quantitative real-time RT-PCR for expression profiling of individual cells."]]
+[[!tag single_cell method PCR inhibition]]
+
+Liss B.
+
+Nucleic Acids Res. 2002 Sep 1;30(17):e89.
+
+Improved quantitative real-time RT-PCR for expression profiling of individual cells.
+
+[[!pmid 12202777 desc="Single cell precipitation protocol, because reverse transcriptase from the cDNA mixture inhibits PCR."]]

diff --git a/biblio/16314311.mdwn b/biblio/16314311.mdwn
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+[[!meta title="PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency."]]
+[[!tag method reverse-transcription PCR inhibition]]
+
+Suslov O, Steindler DA.
+
+Nucleic Acids Res. 2005 Nov 27;33(20):e181.
+
+PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency.
+
+[[!pmid 16314311 desc="Phenol / Chloroform / Isoamyl alcohol (PCI) treatment followed by ethanol precipitation strongly decreases Ct as well as increases the consistencies of the comparisons between different dillutions of the same samples"]]

diff --git a/biblio/16642997.mdwn b/biblio/16642997.mdwn
index 602800450b44f623c42909407d43fbf89b2f6088..e4f80222c6d6a1352e2672cc544d827893e2551a 100644 (file)
--- a/biblio/16642997.mdwn
+++ b/biblio/16642997.mdwn
@@ -1,3 +1,3 @@
 [[!meta title="Microfluidic single-cell mRNA isolation and analysis."]]
-[[!tag microfluidics single-cell]]
+[[!tag microfluidics single_cell]]
 [[!pmid 16642997 desc="The RNA are manipulated with magnetic beads which carry oliognuclotides serving as support and as RT primers."]]

diff --git a/biblio/17071717.mdwn b/biblio/17071717.mdwn
index 8558a03021d0f8b1b5ec46fabbe60a5af0c13903..06041cf1d0d5914fc46e15c27efe8e84bfbf670d 100644 (file)
--- a/biblio/17071717.mdwn
+++ b/biblio/17071717.mdwn
@@ -1,3 +1,3 @@
 [[!meta title="Gene expression profiling of single cells on large-scale oligonucleotide arrays."]]
-[[!tag single-cell amplification oligonucleotides]]
+[[!tag single_cell amplification oligonucleotides]]
 [[!pmid 17071717 desc="High concentration of a dT and random primers mix in reverse transcription."]]

diff --git a/biblio/19363473.mdwn b/biblio/19363473.mdwn
index 20cd88feed3787c937ada253a2a001e55ec37ee9..7813e7f7be36131d8545fd2404b1c81ba8b0ecc8 100644 (file)
--- a/biblio/19363473.mdwn
+++ b/biblio/19363473.mdwn
@@ -1,3 +1,3 @@
 [[!meta title="Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis."]]
-[[!tag single-cell]]
+[[!tag single_cell]]
 [[!pmid 19363473 desc="“Protein state is transmitted from mother to daughter, giving rise to transient heritability in fate, but protein synthesis promotes rapid divergence so that sister cells soon become no more similar to each other than pairs of cells chosen at random.”"]]

diff --git a/biblio/20110323.mdwn b/biblio/20110323.mdwn
index edf4c71f95620b4a42b39be872f784d26148970d..0802394934b66b94f77dcd534c5eced2b836f3d4 100644 (file)
--- a/biblio/20110323.mdwn
+++ b/biblio/20110323.mdwn
@@ -1,3 +1,3 @@
 [[!meta title="Digital nature of the immediate-early transcriptional response."]]
-[[!tag single-cell transcription]]
+[[!tag single_cell transcription]]
 [[!pmid 20110323 desc="Dictyostelium slugs stimulated with cAMP and DIF show a dose-dependent mosaic response."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index 7ac6c309a1ab7db8cebf7bf2a4372e52673c4e44..f178374c0c998626a5698ebbad5197ac03ac553c 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -148,9 +148,6 @@ Ligation of cDNA and methylated oligos, in presence of methy-sensitive restricti
 14513556 [libraries]
 Hybridises single-stranded tester plasmid and driver PCR product, then purified single strand molecules out of duplexes using hydroxyapatite.
 
-12202777 [single cells] [tracked]
-Single cell precipitation protocol, becausr Reverse Transcriptase from the cDNA mixture inhibits PCR.
-
 12161758 [amplification]
 A method to amplify two distinguishable substrates in the same PCR tube, so that they are subjected to the same biases.
 
@@ -822,9 +819,6 @@ Polymerases selected on the ability to amplify their gene despite a 3' mismach i
 7760832 [libraries]
 An eIF-4E fusion protein is used to bind the caps of RNA/DNA duplexes. RNase A cuts single stranded RNAs, thus separating the complexes from the cDNA if they are not full length.
 
-16314311 [enzymes]
-Phenol / Chloroform / Isoamyl alcohol (PCI) treatment followed by ethanol precipitation strongly decreases Ct as well as increases the consistencies of the comparisons between different dillutions of the same samples.
-
 16308152 [amplification]
 Detailed protocol for Terminal Continuation (TC).