--- /dev/null
+[[!meta title="Serial analysis of gene expression in a single cell."]]
+[[!tag sequence_tags single_cell]]
+
+Schober MS, Min YN, Chen YQ.
+
+Biotechniques. 2001 Dec;31(6):1240-2.
+
+Serial analysis of gene expression in a single cell.
+
+[[!pmid 11768647 desc="Lysis in Dynabeads buffer, followed by first strand synthesis from poly-A."]]
--- /dev/null
+[[!meta title="Expression profiling of single cells using 3 prime end amplification (TPEA) PCR."]]
+[[!tag single_cell]]
+
+Dixon AK, Richardson PJ, Lee K, Carter NP, Freeman TC.
+
+Nucleic Acids Res. 1998 Oct 1;26(19):4426-31.
+
+Expression profiling of single cells using 3 prime end amplification (TPEA) PCR.
+
+[[!pmid 9742245 desc="Nucleus removed by spinning. 1st PCR with poly-T and random primer. 2nd PCR nested and gene specific."]]
15271495 [single cell]
Review
-9742245 [single cell] [tracked]
-Nucleus removed by spinning. 1st PCR with poly-T and random primer. 2nd PCR nested and gene specific.
-
15070753 [libraries]
Library of DNAseI ends, to map hypersensitive sites. Substrraction with a HS-depleted tester.
14663149 [libraries] [tracked]
5' 20mers to map transcription start sites, and mesaure their usage.
-11768647 [single cell]
-Lysis in Dynabeads buffer, followed by first strand synthesis from poly-A.
-
15342556 [genome]
These blocks contain most validated and predicted transcription factor binding sites.