bioRxiv

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+[[!meta title="Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting"]]
+[[!tag emulsion Nanopore HPV]]
+
+Esben Bjoern Madsen, Thomas Kvist, Ida Hoijer, Adam Ameur, Marie Just Mikkelsen
+
+bioRxiv, September 6, 2018.
+
+Xdrop: targeted sequencing of long DNA molecules from low input samples using droplet sorting
+
+[[!doi: 10.1101/409086 desc="Enrichment by 1) encapsulating DNA fragments in double-emulsion water-in-oil-in-water droplets, 2) sorting the droplets containing the target sequence detected by a hydrolysis probe PCR, 3) extracting DNA from the droplets, 4) amplifying it by MDA (in a second generation of droplets), and finally 5) sequencing.  When enriching for HPV DNA in HeLa genomes, 5 to 10% of the reads were on target.  Emulsions were prepared in Dolomite chips."]]