+++ /dev/null
-[[!meta title="Purification of histidine-tagged T4 RNA ligase from E. coli."]]
-[[!tag enzyme ligase]]
-
-Biotechniques. 2002 Dec;33(6):1256-60
-
-Wang QS, Unrau PJ.
-
-Purification of histidine-tagged T4 RNA ligase from E. coli.
-
-[[!pmid 12503310 desc="De-adenylation of T4 RNA ligase is required prior using adenylated substrates. Incubating with pyrophosphate during some purification steps does the job. Commercial T4 usually contains mostly de-adenylated enzyme. Mesuring reaction efficiency without ATP gives adenylation levels."]]
--- /dev/null
+[[!meta title="Purification of histidine-tagged T4 RNA ligase from E. coli."]]
+[[!tag enzyme ligase]]
+
+Biotechniques. 2002 Dec;33(6):1256-60
+
+Wang QS, Unrau PJ.
+
+Purification of histidine-tagged T4 RNA ligase from E. coli.
+
+[[!pmid 12503310 desc="De-adenylation of T4 RNA ligase is required prior using adenylated substrates. Incubating with pyrophosphate during some purification steps does the job. Commercial T4 usually contains mostly de-adenylated enzyme. Mesuring reaction efficiency without ATP gives adenylation levels."]]