[[!meta title="CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs."]]
[[!tag cap method enzyme manganese]]
+
+Nucleic Acids Res. 1999 Nov 1;27(21):e31.
+
+Schmidt WM, Mueller MW.
+
+CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs.
+
[[!pmid 10518626 desc="In presence of the 5' cap and Mn2+, the SuperScriptII enzyme adds 3-4 extra dC residues to the first strand cDNA."]]
[[!meta title="pages tagged cap"]]
-[[!inline pages="tagged(cap)" actions="no" archive="yes"
-feedshow=10]]
+Methods for enriching capped RNAs.
+
+(work in progress)
+
+ - [[CapSelect|biblio/10518626.mdwn]]: Add a poly-A tail to the first-strand
+ cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG
+ overhang, and prime second-strand synthesis.
+
+[[!inline pages="tagged(cap)" limit=0]]