re-read

diff --git a/biblio/10471753.mdwn b/biblio/10471753.mdwn
index 495da5a4871500d8d1c942c004822249167b4837..fc24caa9c8cda1b15333801412cfe4e4357a6e30 100644 (file)
--- a/biblio/10471753.mdwn
+++ b/biblio/10471753.mdwn
@@ -7,6 +7,7 @@ Shagin DA, Lukyanov KA, Vagner LL, Matz MV.
 
 Regulation of average length of complex PCR product.
 
-[[!pmid 10471753 desc="Amplify preferentially long PCR product with a single
-primer. Shorter molecules have a higher probability of undergoing
-inhibitory intramolecular interactions. (suppressive PCR)"]]
+[[!pmid 10471753 desc="Suppression PCR amplification of a phage lambda digested
+by HindII on which inverted terminal repeats were ligated.  Suppression effect
+is stronger when the PCR primer is shorter than the terminal repeats, and
+when the PCR primer molarity is lower (tested in a 750–0 nM range)."]]

diff --git a/tags/amplification.mdwn b/tags/amplification.mdwn
index 531fee7123492a379a4b2181473df7aa136abdad..798fcbaf55106621f0d61f795259abf5ccb85718 100644 (file)
--- a/tags/amplification.mdwn
+++ b/tags/amplification.mdwn
@@ -5,6 +5,10 @@ _work in progress..._
 ‘Suppression PCR’ was first published in English in [[Siebert and coll.,
 1995|biblio/7731798]].  Figure 1B shows a ‘panhandle’ structure.
 
+Primer concentration (lower -> stronger suppression) and length ratio between
+inverted tandem repeats and PCR primer (shorter PCR primer -> stronger
+suppression) were investigated by Shagin and coll ([[1999|biblio/10471753]]).
+
 Suppression PCR usually does not affect long (6~8 kbp) DNA molecules
 (mentionned in [[Lukyanov and coll., 2007|biblio/10.1007_978-1-4020-6040-3_2]]).