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diff --git a/biblio/3799962.mdwn b/biblio/3799962.mdwn
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+++ b/biblio/3799962.mdwn
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+[[!meta title="Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase."]]
+[[!tag ligase]]
+
+Anal Biochem. 1986 Oct;158(1):171-8.
+
+Tessier DC, Brousseau R, Vernet T.
+
+Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase.
+
+[[!pmid 3799962 desc="ssDNA ligation. Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM."]]

diff --git a/biblio/3978074.mdwn b/biblio/3978074.mdwn
index b43d50867fcf3b6fef24bc982f01d126a2c105e9..718b8580493e1bd78a4dd7c7fb5e7c72c16a776c 100644 (file)
--- a/biblio/3978074.mdwn
+++ b/biblio/3978074.mdwn
@@ -7,4 +7,4 @@ Biochemistry. 1985 Jan 15;24(2):267-73.
 
 Donor activation in the T4 RNA ligase reaction.
 
-[[!pmid 3978074 desc="Adenylated RNA is a better substrate for T4 RNA ligase."]]
+[[!pmid 3978074 desc="Adenylated RNA is a better substrate for T4 RNA ligase.  Adenylation efficiency after 6h: C >> U ~ A > G."]]

diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn
index 6fc507cc2deaa4a3ba571a0ddd8f6c5c21052abd..afc1dd1550256b83b87ce570ff6792d6afda95ad 100644 (file)
--- a/tags/ligase.mdwn
+++ b/tags/ligase.mdwn
@@ -2,10 +2,18 @@
 
 Work in progress.
 
+Ligases are inhibited by high concentrations of ATP ([[Tessier, Brousserau &
+Vernet, 1986|biblio/3799962]] and many others).
+
 On T4 RNL1:
 
  - Using poly-A as substrate for circularisation, minimal length is 8, optimal
    is 10, and then efficiency decreases slowly ([[Kaufmann, Klein & Littauer, 1974|biblio/4609429]]).
+ - Adenylated RNA is a better substrate for T4 RNA ligase.
+   Adenylation efficiency after 6h: C >> U ~ A > G ([[McLaughlin et al., 1985|biblio/3978074]]).
+ - Also used for single-strand DNA ligation ([[Tessier, Brousserau & Vernet, 1986|biblio/3799962]])
+   with PEG and hexamine cobalt chloride (HCC) as additives.
+
 
 On T4 RNL2: