Bonané

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+[[!meta title="Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning."]]
+[[!tag library exonuclease method]]
+
+Biotechniques. 2017 Sep 1;63(3):125-130. doi:10.2144/000114588
+
+Islam MN, Lee KW, Yim HS, Lee SH, Jung HC, Lee JH, Jeong JY.
+
+Optimizing T4 DNA polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning.
+
+[[!pmid 28911316 desc="Use of T4 DNA polymerase at 50 °C as an exonuclease for generating sinngle-strand overhangs."]]