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+[[!meta title="Gene duplications and the origins of vertebrate development."]]
+[[!tag Oikopleura]]
+
+Holland PW, Garcia-Fernàndez J, Williams NA, Sidow A.
+
+Dev Suppl. 1994:125-33.
+
+Gene duplications and the origins of vertebrate development.
+
+[[!pmid 7579513 desc="Attempt to clone Hox genes with degenerate primers only yielded one clone."]]

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
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--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -174,6 +174,8 @@ Genes and pathways
    are activated by polyunsaturated aldehydes produced by diatoms
    ([[Torres-Águila and coll., 2018|biblio/30272001]]).
  - _O. dioica_ has 83 homeobox genes, according to [[Edvardsen and coll., 2005|biblio/15649342]].
+   Illustrating how diverged are the sequences, earlier attempts to clone Hox genes yielded
+   only a single clone ([[Holland and coll. 1994|biblio/7579513]]).
  - Southern blot analysis suggest the presence of a SCO-spondin gene in _O.
    dioica_ ([[Gobron and coll., 1999|biblio/10197783]]).
  - A single ortholog of _Brachyury_ (_TBXT_, or _T_) was cloned by [[Bassham