Café

diff --git a/biblio/33423048.mdwn b/biblio/33423048.mdwn
new file mode 100644 (file)
index 0000000..270356b
--- /dev/null
+++ b/biblio/33423048.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae."]]
+[[!tag synthetic yeast chromosome]]
+
+Postma ED, Dashko S, van Breemen L, Taylor Parkins SK, van den Broek M, Daran JM, Daran-Lapujade P.
+
+Nucleic Acids Res. 2021 Feb 22;49(3):1769-1783. doi:10.1093/nar/gkaa1167
+
+A supernumerary designer chromosome for modular in vivo pathway assembly in _Saccharomyces cerevisiae_.
+
+[[!pmid 33423048 desc="Circular neochromosome assembled in vivo by homologous recombination of fragments containing short homology regions (60 bp).  Includes “a centromere and autonomously replicating sequences (ARS) spaced every 30–40 kb, and markers to facilitate [...] selection”.  Used as “landing pads” for integration of ~30kb-scale fragments, using CRISPR/Cas9 to cleave the integration site.  The neochromosomes can contain entire metabolic pathways, either endogenous (after knocking-out the endogenous copies) or exogenous."]]

diff --git a/tags/synthetic.mdwn b/tags/synthetic.mdwn
index c1548515abaa4c718494b161b7ebbd64376f2851..06d8d9a349bd640d9b30b4365af6e99ce56b8182 100644 (file)
--- a/tags/synthetic.mdwn
+++ b/tags/synthetic.mdwn
@@ -6,4 +6,6 @@ Sc3.0 roadmap: [[Dai and coll., 2020|biblio/32791980]].
 
 “Biosynthesis of medicinal tropane alkaloids in yeast”: [[Srinivasan and Smolke and coll., 2020|biblio/32879484]]
 
+Example of neochromosome synthesis in yeast: [[Postma and coll., 2021|biblio/33423048]].
+
 [[!inline pages="tagged(synthethic)" limit=0]]