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diff --git a/biblio/10.1007_s00227-022-04145-5.mdwn b/biblio/10.1007_s00227-022-04145-5.mdwn
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+[[!meta title="The cosmopolitan appendicularian Oikopleura dioica reveals hidden genetic diversity around the globe."]]
+[[!tag Oikopleura OIST]]
+
+Aki Masunaga, Michael J. Mansfield, Yongkai Tan, Andrew W. Liu, Aleksandra Bliznina, Paolo Barzaghi, Tamara L. Hodgetts, Alfonso Ferrández-Roldán, Cristian Cañestro, Takeshi A. Onuma, Charles Plessy & Nicholas M. Luscombe.
+
+The cosmopolitan appendicularian Oikopleura dioica reveals hidden genetic diversity around the globe.
+
+[[!doi 10.1007/s00227-022-04145-5 desc="Three (cryptic) _O. dioica_ species.  The only robust morphological change found was egg diameter."]]

diff --git a/biblio/38621828.mdwn b/biblio/38621828.mdwn
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+[[!meta title="Extreme genome scrambling in marine planktonic Oikopleura dioica cryptic species."]]
+[[!tag Oikopleura OIST]]
+
+Plessy C, Mansfield MJ, Bliznina A, Masunaga A, West C, Tan Y, Liu AW, Grašič J, Del Río Pisula MS, Sánchez-Serna G, Fabrega-Torrus M, Ferrández-Roldán A, Roncalli V, Navratilova P, Thompson EM, Onuma T, Nishida H, Cañestro C, Luscombe NM.
+
+Genome Res. 2024 Apr 25;34(3):426-440. doi:10.1101/gr.278295.123
+
+Extreme genome scrambling in marine planktonic Oikopleura dioica cryptic species. 
+
+[[!pmid 38621828 desc="Gene order is scrambled between _O. dioica_ species.  Molecular clock analysis of 177 single-copy orthologs places appendicularian sister to all tunicates."]]

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 5d18ecf71af1f86caaa4cea4a84b0c0a1e4dd635..bac98b529d8bb7fd75418420df3d24fc984b6113 100644 (file)
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -57,10 +57,16 @@ Phylogeny
    sister to all other tunicates.
  - Study of 117 phenotypic characters in 49 tunicate species also support basal
    position of appendicularians ([[Braun, Leubner and Stach, 2019|biblio/10.1111_cla.12405]].
- - “The difference between coding sequences was considerably higher in
-   comparisons between strains of different oceans than within the Bergen gene
-   pool.  We ignore whether and how Oikopleura dioica is subdivided into multiple
-   species” ([[Denoeud et al., 2010|biblio/21097902]]).
+ - Molecular clock analysis of 177 single-copy orthologs also places appendicularians
+   sister to all tunicates ([[Plessy, Mansfield and coll., 2024|biblio/38621828]]).
+ - _O. dioica_ is actually mutliple species.  “The difference between coding
+   sequences was considerably higher in comparisons between strains of
+   different oceans than within the Bergen gene pool.  We ignore whether and how
+   Oikopleura dioica is subdivided into multiple species” ([[Denoeud et al.,
+   2010|biblio/21097902]]).  Masunaga and coll. ([[2022|biblio/10.1007_s00227-022-04145-5]])
+   demonstrated the existence of at least 3 species using molecular markers, and
+   found that egg diameter distinguishes the “okinawan” one from the others.
+   Gene order is scrambled between the 3 species ([[Plessy, Mansfield and coll., 2024|biblio/38621828]]).
  - Giant Oikopleurid species, such as  exist in deeper waters. _Bathochordaeus charon_'s 18S
    RNA is 97% identical to the one of _O. dioica_ ([[Sherlock and coll, 2016|biblio/10.1186_s41200-016-0075-9]]).
  - CO1 DNA of _B. mcnutti_ and _B. strygius_ are ~12% different ([[Sherlock and coll., 2017|biblio/28042175]]).