--- /dev/null
+[[!meta title="Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase."]]
+[[!tag ligation method]]
+
+Nucleic Acids Res. 1985 Mar 25;13(6):1997-2008.
+
+Rusche JR, Howard-Flanders P.
+
+Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase.
+
+[[!pmid 4000951 desc="HCC (1 mM) promotes intermolecular ligation. This effect is countered by monovalent cations."]]
12448877 [protocols]
Ligating a DNA adaptor 3' to the FScDNA using T4RNA ligase.
-4000951 [protocols]
-HCC (1 mM) promotes intermolecular ligation. This effect is countered by monovalent cations.
-
386284 [protocols]
A low ATP concentration contributes to the attainment of high yields. ATP regenerating systems allow to work at sub-stoechiometric concentrations. Rnase A can be used as a SSBP. Spermine enhances yield.