Au bureau.

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+[[!meta title="Single-site DNA cleavage by Type III restriction endonuclease requires a site-bound enzyme and a trans-acting enzyme that are ATPase-activated."]]
+[[!tag EcoP15I]]
+
+Ahmad I, Kulkarni M, Gopinath A, Saikrishnan K.
+
+Nucleic Acids Res. 2018 Jul 6;46(12):6229-6237. doi:10.1093/nar/gky344
+
+Single-site DNA cleavage by Type III restriction endonuclease requires a site-bound enzyme and a trans-acting enzyme that are ATPase-activated.
+
+[[!pmid 29846668 desc="Single-site cleavage happens by cooperation between activated enzymes contacting each other by 3D diffusion, and therefore requires higher concentrations of enzyme than two-site cleavage, where contacts happen through 1D diffusion or looping."]]