- A method similar to oligo-capping was reported by [[Sekine and Kato|biblio/8247743]] in 1993.
- - Oligo-capping [[Suzuki et al., 1999|biblio/9373149]]: dephosphorylate, uncover functional
- phosphate with decapping enzyme, and ligate RNA adaptor with T4 RNA ligase.
+ - Oligo-capping ([[Suzuki et al., 1999|biblio/9373149]]): dephosphorylate,
+ uncover functional phosphate with decapping enzyme, and ligate RNA adaptor
+ with T4 RNA ligase.
(wip: Fromont-racine et al., Maruyama et al.)
- - CapSelect [[Schmidt et al., 1999|biblio/10518626]]: Add a poly-A tail to the first-strand
- cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG
- overhang, and prime second-strand synthesis.
+ - CapSelect ([[Schmidt et al., 1999|biblio/10518626]]): Add a poly-A tail to
+ the first-strand cDNA, ligate a double-stranded adapter ending with a
+ single-strand (T)TTTGGG overhang, and prime second-strand synthesis.
- - [[Cleptet et al., 2004|biblio/14704363]] used oligo-capping, but used T4 DNA ligase and a double-stranded
- adapter with NNNNNN overhang instead of T4 RNA ligase and a single-stranded linker.
+ - [[Cleptet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4
+ DNA ligase and a double-stranded adapter with NNNNNN overhang instead of T4
+ RNA ligase and a single-stranded linker.
[[!inline pages="tagged(cap)" limit=0]]