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+[[!meta title="Fast and accurate de novo genome assembly from long uncorrected reads."]]
+[[!tag genome assembly software]]
+
+Vaser R, Sović I, Nagarajan N, Šikić M.
+
+Genome Res. 2017 May;27(5):737-746. doi:10.1101/gr.214270.116
+
+Fast and accurate de novo genome assembly from long uncorrected reads.
+
+[[!pmid 28100585 desc="Racon can be used to correct contigs or to correct raw reads."]]
The Flye assembler ([[Kolmogorov and coll, bioRxiv
2018|biblio/10.1101_247148]]) creates an A-Bruijn (assembly) graph from draft
contigs using long error-prone reads, untangles the graph by resolving repeats,
-and then uses it to refine the contings and increase their accuracy.
+and then uses it to refine the contings and increase their accuracy. (The
+predecessor of Flye, ABruijn, was reported by [[Istace and coll.
+(2017)|biblio/28369459]] to be able to assemble mitochondrial genomes, unlike
+Canu and other assemblers.)
-The predecessor of Flye, ABruijn, was reported by [[Istace and coll.
-(2017)|biblio/28369459]] to be able to assemble mitochondrial genomes (unlike
-Canu and other assemblers).
+After assembly, the contigs can be further polished with Racon ([[Vaser, Sović,
+Nagarajan and Šikić, 2017|biblio/28100585]]).
When coverage is too low for efficient reference-free assembly, related
references can be used as a guide. The Ragout software ([[Kolmogorov and