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@@ -0,0 +1,10 @@
+[[!meta title="3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM."]]
+[[!tag Oikopleura]]
+
+3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM.
+
+Nishida H, Ohno N, Caicci F, Manni L.
+
+Sci Rep. 2021 Mar 1;11(1):4833. doi:10.1038/s41598-021-83706-y
+
+[[!pmid 33649401 desc="“The bilateral arrangement of precursor cells of the oikoplastic epidermis was already attained by the hatching stage.”  “Upon 3D construction, additional single cells, which showed similar features to giant Fol cells, albeit slightly smaller, were present in the lateral part. It is possible that this cell will be lost after larval development.”  “The gonad syncytium formation already starts as early as in 10 hpf juveniles by the cell fusion of germ cells, although it is not possible to discern males or females at this stage.”"]]

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index c29ff6258b3c9ebd1eda5b4dc3d67e0f07fc72f0..2c1b15c3cf40c5018b9a441fa2ae1613f3a5678a 100644 (file)
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -332,6 +332,7 @@ Development
  - The oocytes originate from a specialised syncitium, the coenocyst, in which
    nurse cells and oocytes are connected by cytoplasmic bridges, the ring channels
    ([[Ganot and coll., 2007|biblio/17126826]]).
+ - The gonad syncytium forms as early at 10 hpf ([[Nishida and coll., 2021|biblio/33649401]]).
  - Oocytes lacking odCDK1d can not resume from meiosis from prophase I arrest,
    and therefore are non-viable after spawning ([[Øvrebø and coll., 2015|biblio/25714331]]).
  - Oocytes are in metaphase I stage at the time of spawning ([[Ganot, Kallesøe
@@ -392,7 +393,8 @@ Development
  - The oral gland precursor is a syncytium with 4 nuclei that migrates
    anteriorly.  The two differentiated oral gland cells have two nuclei each,
    as demonstrated by a co-staining of nuclei (H2B-mCherry) and cell membrane
-   (PH-YF) by [[Kishi and coll, 2014|biblio/25224225]].
+   (PH-YF) by [[Kishi and coll, 2014|biblio/25224225]], as well as SEM tomography
+   ([[Nishida and coll., 2021|biblio/33649401]]).
  - Oral gland and subchordal cells, which were thought to be related, do not originate
    from the same blastomere ([[Onuma and coll., 2020|biblio/32029598]]).
  - The subchordal cell precursors migrate along the right side of the notochord
@@ -431,6 +433,8 @@ Anatomy
    in the central canal ([[Holmberg and Olsson, 1984|biblio/reissner_oik]]).
  - In contrary to Kowalevskiidae, ([[Brena, Cima and Burighel, 2003|biblio/10.1002_jmor.10145]]),
    _Oikopleura_ do have a heart.
+ - A 3D reconstitution of hatchlings and jufeniles was done by SEM tomography by
+   [[Nishida and coll., 2021|biblio/33649401]].
 
 Physiology
 ----------
@@ -486,6 +490,9 @@ House
    1985|biblio/10.2307_1541178]]).  In these species, light is produced by
    granular inclusions in the house.  In species without oral glands,
    bioluminescence might be caused by dinoflagellates.
+ - A 8th cell was seen in the Fol area at 10 hpf in the 3D tomography analysis
+   of [[Nishida and coll., 2021|biblio/33649401]].  It is possible that this cell
+   is lost during later development.
 
 
 Phenotypes