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diff --git a/biblio/14704363.mdwn b/biblio/14704363.mdwn
new file mode 100644 (file)
index 0000000..6816e33
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+++ b/biblio/14704363.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Improved full-length cDNA production based on RNA tagging by T4 DNA ligase."]]
+[[!tag cap method]]
+
+Nucleic Acids Res. 2004 Jan 2;32(1):e6 doi:10.1093/nar/gng158
+
+Clepet C, Le Clainche I, Caboche M.
+
+Improved full-length cDNA production based on RNA tagging by T4 DNA ligase.
+
+[[!pmid 14704363 desc="T4 DNA ligase used to ligate a double stranded cap-tag."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index fbc7431763399c3b9f919d887c664f49dddc12ed..8db5444ddaf9d60a6251ad25e50690e54c0b8156 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -80,9 +80,6 @@ Oligo-capping to build 5' ou FL cDNA libraries.
 14606961 [libraries]
 The nuclease activity of T7 RNA pol could also show some transcript specificity.
 
-14704353 [libraries] [tracked]
-T4 DNA ligase used to ligate a double stranded cap-tag.
-
 3799962 [enzymes]
 Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM.
 

diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index b21b128192c30b4d4094c59e7a3c223693210a0b..cc9b7317011eb516695fa9c2f15c77ad7e54cdcd 100644 (file)
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -4,8 +4,11 @@ Methods for enriching capped RNAs.
 
 (work in progress)
 
- - [[CapSelect|biblio/10518626]]: Add a poly-A tail to the first-strand
+ - CapSelect [[Schmidt et al., 1999|biblio/10518626]]: Add a poly-A tail to the first-strand
    cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG
    overhang, and prime second-strand synthesis.
 
+ - [[Cleptet et al., 2004|biblio/14704363]] used oligo-capping, but used T4 DNA ligase and a double-stranded
+   adapter with NNNNNN overhang instead of T4 RNA ligase and a single-stranded linker.
+
 [[!inline pages="tagged(cap)" limit=0]]