C'était l'heure de la soupe.

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+[[!meta title="Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions."]]
+[[!tag DTT reverse_transcription qPCR]]
+
+Lekanne Deprez RH, Fijnvandraat AC, Ruijter JM, Moorman AF.
+
+Anal Biochem. 2002 Aug 1;307(1):63-9.
+
+Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions.
+
+[[!pmid 12137780 desc="DTT interferes with fluorescence measurements in qPCR.  RT without DTT is efficient.  Random-primerd replicates are less reproducible than oligo-dT or gene-specific-primed ones."]]