[[!meta title="Amplification of cDNA ends based on template-switching effect and step-out PCR."]]
[[!tag template_switching PCR method ]]
+
+Nucleic Acids Res. 1999 Mar 15;27(6):1558-60.
+
+Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A.
+
+Amplification of cDNA ends based on template-switching effect and step-out PCR.
+
[[!pmid 10037822 desc="To counter template-switching happening from inside the oligonucleotide instead of its tail."]]
Amine-modified random primers to label probes for DNA microarrays.
-[[!pmid desc="Incorporates aminoallyl-dUTP instead of cy3/5dUTP. Uses priming hexamers with a amino C6dT in 5′. This allows to reduce by 10 folds the quantity of RNA as a starting material. Presence of amplification products of t/rRNAs is not a problem when « optimal » quantities of total RNA are used."]]
+[[!pmid 12089562 desc="Incorporates aminoallyl-dUTP instead of cy3/5dUTP. Uses priming hexamers with a amino C6dT in 5′. This allows to reduce by 10 folds the quantity of RNA as a starting material. Presence of amplification products of t/rRNAs is not a problem when « optimal » quantities of total RNA are used."]]