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+[[!meta title="Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq."]]
+[[!tag library method]]
+
+Yan B, Tzertzinis G, Schildkraut I, Ettwiller L.
+
+Genome Res. 2021 Nov 23. doi:10.1101/gr.275784.121
+
+Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq.
+
+[[!pmid 34815308 desc="5 µg of total RNAs was decapped with the the yeast scavenger decapping enzyme (yDcpS), and recapped with vaccinia capping enzyme (VCE) and a biotinylated guanosine.  Therefore the protocol enriches for capped, triphosphorylated, diphosphorylated, but not monophosphorylated RNAs.  A control library made on RNA dephosphorylated with CIP was used to infer if a TSS is driven by Pol II or Pol III.  The methyl-triphosphate cap of RN7SK resists to the CIP treatement and causes it to be incorrectly classified Pol II."]]