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+[[!meta title="Cell culture-based shark karyotyping as a resource for chromosome-scale genome analysis"]]
+[[!tag bioRxiv cell_culture karyotype]]
+
+Yoshinobu Uno, Ryo Nozu, Itsuki Kiyatake, Nobuyuki Higashiguchi, Shuji Sodeyama, Kiyomi Murakumo, Keiichi Sato, Shigehiro Kuraku
+
+bioRxiv 2020.09.08.286724; doi: https://doi.org/10.1101/2020.09.08.286724
+
+Cell culture-based shark karyotyping as a resource for chromosome-scale genome analysis
+
+[[!doi 10.1101/2020.09.08.286724 desc="Primary cell culture of fibroblasts and lymphocytes.  Mitogens were used to stimulate lymphocyte growth. “2n = 102 for the whale shark (Rhincodon typus) and zebra shark (Stegostoma fasciatum), and 2n = 106 for the brownbanded bamboo shark (Chiloscyllium punctatum) and whitespotted bamboo shark (C. plagiosum)”.  In comparison with teleost cell culture medium, shark cells needed a higher osmolarity and the medium was supplemented with 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide."]]