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+[[!meta title="cDNA library construction from a small amount of RNA: adaptor-ligation approach for two-round cRNA amplification using T7 and SP6 RNA polymerases."]]
+[[!tag ]]
+
+Biotechniques. 2005 Mar;38(3):451-8.
+
+Ohara R, Kikuno RF, Kitamura H, Ohara O.
+
+cDNA library construction from a small amount of RNA: adaptor-ligation approach for two-round cRNA amplification using T7 and SP6 RNA polymerases.
+
+[[!pmid 15786810 desc="High size is preserved by ligating a 5'adapter before each amplification."]]
15785770 [misc]
Future Nobel prize ? Maybe an explanation for the conservation of dev. enhancers.
-15786810 [libraries]
-High size is preserved by ligating a 5'adapter before each amplification.
-
15778292 [genome]
2n species covered 1x would be more efficient than n covered 2x for the discovery of conserved elements, but the resulting genome assemblies would be of a much poorer qualities.