--- /dev/null
+[[!meta title="Gene discovery in genetically labeled single dopaminergic neurons of the retina."]]
+[[!tag single_cell library]]
+
+Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):5069-74. doi:10.1073/pnas.0400913101
+
+Gustincich S, Contini M, Gariboldi M, Puopolo M, Kadota K, Bono H, LeMieux J, Walsh P, Carninci P, Hayashizaki Y, Okazaki Y, Raviola E.
+
+Gene discovery in genetically labeled single dopaminergic neurons of the retina.
+
+[[!pmid 15047890 desc="Uses SMART7, PCR and multiple aRNA rounds."]]
--- /dev/null
+[[!meta title="An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture)."]]
+[[!tag cap libraries]]
+
+Mol Cell Biol. 1995 Jun;15(6):3363-71.
+
+Edery I, Chu LL, Sonenberg N, Pelletier J.
+
+An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture).
+
+[[!pmid desc="An eIF-4E fusion protein is used to bind the caps of RNA/DNA duplexes. RNase A cuts single stranded RNAs, thus separating the complexes from the cDNA if they are not full length."]]
10499525 [single cell]
Single cell RT-PCR.
-15047890 [single cell]
-Uses SMART7, PCR, and multiple aRNA rounds.
-
12711698 [amplification] [tracked]
Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones).
15156154 [emulsion]
Polymerases selected on the ability to amplify their gene despite a 3' mismach in the primers were shown to be able to incorporate a broad range of nucleotide analogs.
-7760832 [libraries]
-An eIF-4E fusion protein is used to bind the caps of RNA/DNA duplexes. RNase A cuts single stranded RNAs, thus separating the complexes from the cDNA if they are not full length.
-
16308152 [amplification]
Detailed protocol for Terminal Continuation (TC).