MthRnl

diff --git a/biblio/18829718.mdwn b/biblio/18829718.mdwn
new file mode 100644 (file)
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+++ b/biblio/18829718.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA."]]
+[[!tag enzyme ligase adenylylation]]
+
+Torchia C, Takagi Y, Ho CK.
+
+Nucleic Acids Res. 2008 Nov;36(19):6218-27. doi:10.1093/nar/gkn602
+
+Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA.
+
+[[!pmid 18829718 desc="Used in the NEB's 5´ DNA Adenylation Kit.  Lacks specificity for RNA and DNA."]]

diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn
index baa0d3c7b98ec41c1c4a61fec5d7b8ecb8aefbd7..2ba035e9f2f934ac6439cc451125c9bb26ab5c1a 100644 (file)
--- a/tags/ligase.mdwn
+++ b/tags/ligase.mdwn
@@ -13,7 +13,7 @@ On T4 RNL1:
    Adenylation efficiency after 6h: C >> U ~ A > G ([[McLaughlin et al., 1985|biblio/3978074]]).
  - Also used for single-strand DNA ligation ([[Tessier, Brousserau & Vernet, 1986|biblio/3799962]])
    with PEG and hexamine cobalt chloride (HCC) as additives.
- - Can dephoshporylate RNA 3′ ends ([[Krug & Uhlenbeck, 1982|biblio/7082652]]).  
+ - Can dephoshporylate RNA 3′ ends ([[Krug & Uhlenbeck, 1982|biblio/7082652]]).
 
 On T4 RNL2:
 
@@ -37,12 +37,19 @@ DNA and RNA ligases can be used to adenylylate a substrate.
  - [[Hoffmann and McLaughlin (1987)|biblio/3299268]] used T4 RNL 1 to
    adenylylate RNA, and noted that ssNpR(A) are good acceptors, and pCpN
    are good donors.
+ - [[Wang and Unrau (2002)|biblio/12503310]] noted that purified
+   recombinant ligase can be partly adenylylated, which blocks the
+   reaction on pre-adenylylated substrates.  Pre-incubation with
+   pyrophosphate can displace AMP moiety.  Commercial ligases
+   are reported to contain mostly non-adenylylated proteins.
  - [[Ho, Wang, Lima and Shuman (2004)|biblio/14962393]] reported the
    use of RNL(1-249) on pre-adenylylated RNA in absence of ATP for
    avoiding concatemerisation.
  - [[Silverman (2004)|biblio/15037782]] reported the use a complementary
    DNA oligonucleotide (leaving 3-4 protruding 5'ribonucleotides) when
    adenylylating with T4 RNL 1.
+ - [[Torchia, Takagi and Ho (2008)|biblio/18829718]] reported the use
+   of the Methanobacterium RNA ligase (MthRnl) to adenylylate RNA.
 
 [[!inline pages="tagged(ligase)" limit=0]]
 [[!meta title="pages tagged adenylylation"]]