ActD.

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 7d2e930a37e5d891a40f412c92c3c8a3d3382ef1..04109217ed70b4e71fe681193dc23d43fee5b863 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -1,4 +1,12 @@
 [[!meta title="pages tagged reverse transcription"]]
 
-[[!inline pages="tagged(reverse_transcription)" actions="no" archive="yes"
-feedshow=10]]
+(redaction in progress)
+
+Reverse-transcriptases have a DNA-dependent DNA polymerase activity.
+ - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.
+ - It is also a source of antisense artefacts when the RT makes a second-strand cDNA
+   that is mistaken for a first-strand cDNA.  ActinomycinD inhibits DNA-dependent,
+   but not RNA-dependent polymerase activity and is used to suppress these
+   artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]).
+
+[[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]]