adenylylation

diff --git a/tags/adenylylation.mdwn b/tags/adenylylation.mdwn
index a33771e835a693d1d897eabef4b5dd226fbb9b32..3a77ffabdf23f5319f22a98a3ae8f7cf96c61848 100644 (file)
--- a/tags/adenylylation.mdwn
+++ b/tags/adenylylation.mdwn
@@ -3,7 +3,7 @@
 Adenylylation
 =============
 
-Adenylylation is the addition of an AMP moiety to a phosphorylated end.  In vitro,
-it is performed by [[ligases|ligases]].
+Adenylylation is the addition of an AMP moiety to a phosphorylated end.  In
+vitro, it is performed by ligases.  See the [[ligase]] tag for details.
 
 [[!inline pages="tagged(adenylylation)" limit=0]]

diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn
index 0fd14bfcb3042292b4c4cf7bed37b1578b1e483f..baa0d3c7b98ec41c1c4a61fec5d7b8ecb8aefbd7 100644 (file)
--- a/tags/ligase.mdwn
+++ b/tags/ligase.mdwn
@@ -2,9 +2,6 @@
 
 Work in progress.
 
-DNA and RNA ligases can be used to adenylylate a substrate; seee the
-[[adenylylation]] tag for details.
-
 Ligases are inhibited by high concentrations of ATP ([[Tessier, Brousserau &
 Vernet, 1986|biblio/3799962]] and many others).
 
@@ -32,4 +29,23 @@ On T4 RNL2:
    linker to RNA ends, thus prevents unwanted ligation products
    ([[Viollet et al., 2011|biblio/21722378]]).
 
+DNA and RNA ligases can be used to adenylylate a substrate.
+
+ - [[McLaughlin, Piel and Graeser (1985)|biblio/3978074]] noted that
+   adenylylated RNA is a better substrate for T4 RNA ligase, and that
+   adenylylation efficiency after 6h was C » U ~ A > G. 
+ - [[Hoffmann and McLaughlin (1987)|biblio/3299268]] used T4 RNL 1 to
+   adenylylate RNA, and noted that ssNpR(A) are good acceptors, and pCpN
+   are good donors.
+ - [[Ho, Wang, Lima and Shuman (2004)|biblio/14962393]] reported the
+   use of RNL(1-249) on pre-adenylylated RNA in absence of ATP for
+   avoiding concatemerisation.
+ - [[Silverman (2004)|biblio/15037782]] reported the use a complementary
+   DNA oligonucleotide (leaving 3-4 protruding 5'ribonucleotides) when
+   adenylylating with T4 RNL 1.
+
 [[!inline pages="tagged(ligase)" limit=0]]
+[[!meta title="pages tagged adenylylation"]]
+
+
+[[!inline pages="tagged(adenylylation)" limit=0]]