[[!meta title="High-accuracy amplification of nanogram total RNA amounts for gene profiling."]]
-[[!tag enzyme ssbp]]
+[[!tag reverse_transcription ssbp]]
+
+Kenzelmann M, Klären R, Hergenhahn M, Bonrouhi M, Gröne HJ, Schmid W, Schütz G.
+
+Genomics. 2004 Apr;83(4):550-8 doi:10.1016/j.ygeno.2003.09.026
+
+High-accuracy amplification of nanogram total RNA amounts for gene profiling.
+
[[!pmid 15028277 desc="Augment temperature, lower primer concentration, and add T4gp32 to enhance reverse transcription."]]
[[!meta title="Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein."]]
-[[!tag reverse_transcription]]
+[[!tag reverse_transcription enzyme ssbp]]
J Biomol Tech. 2005 Sep;16(3):239-47
Additives that increase reaction performance:
- Actinomycin D ([[Perocchi et al., 2007|biblio/17897965]]).
- - T4 bacteriophage gene 32 protein (T4gp32, [[Piché _et al._, 2005|biblio/16461948]]).
+ - T4 bacteriophage gene 32 protein (T4gp32, [[Kenzelmann _et al._, 2004|biblio/15028277]], [[Piché _et al._, 2005|biblio/16461948]]).
Reverse-transcriptases have a DNA-dependent DNA polymerase activity.
- It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.