Café

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+[[!meta title="Establishing Sustainable Cell Lines of a Coral, Acropora tenuis."]]
+[[!tag coral cell_culture cell_line]]
+
+Kawamura K, Nishitsuji K, Shoguchi E, Fujiwara S, Satoh N.
+
+Mar Biotechnol (NY). 2021 Apr 26. doi:10.1007/s10126-021-10031-w
+
+Establishing Sustainable Cell Lines of a Coral, Acropora tenuis.
+
+[[!pmid  33899125 desc="Dissociation with “a mixture of trypsin, EDTA, and collagenase”.  “Treatment for 1–2 h at 25–28 °C did not complete cell dissociation, but after 3–4 h, only single cells without debris were found in the culture dish.”  “[The modular protease] plasmin (2 μg/mL) was effective in maintaining dissociated cells in culture for more than 2 weeks, during which a large number of a new type of cell appeared in the culture dish.”  “Aliquots of polyclonal cell population were harvested from the primary 24-well culture plate, diluted fivefold with growth medium containing plasmin, and dispensed into 96-well plates (100 μL/well). Cells in clumps proliferated with a doubling time of 2–3 days.”"]]

diff --git a/tags/cell_line.mdwn b/tags/cell_line.mdwn
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@@ -4,5 +4,6 @@ A few notes that just scratch the surface of a vast field…
 
  - [[Echalier and Ohanessian (1969)|biblio/4976834]] reported a culture of _Drosophila_ cells that spontaneously transformed.
  - [[Schneider's (1972)|biblio/4625067]] report of the establishment of the S2 cell line.
+ - [[Kawamura and coll (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines.
 
 [[!inline pages="tagged(cell_line)" limit=0]]